Rutin

1) NF11 Appearance: yellow to greenish yellow crystalline powder Identification: (A), (B), (C), (D), ( E ) positive reactions Assay: 95.0%min Loss on drying: 5.5-9.0% Residue on ignition: 0.5%max Chlorophyll: 0.004%max Red pigment: 0.004%max Quercetin: 0.5%max Microbiological tests: Total plate count/1g: 1000max Yeast and mould/1g: 100max E. Coil/1g: Absence Package: 25kg/ fibre drum 2) EP9 Appearance: Yellow or greenish-yellow powder Identification: First identification: B Second identification: A. C. D -A (UV/ Vis absorption of methanol solution between 210nm and 450nm ) ---- 2maxima at 257nm and 358nm , specific absorbance at 358nm is 305-330nm -B (IR) ---- Comparison : rutoside trihydrate CRS -C (TLC) --- Principle spot is similar to that of reference solution -D ---- Dissolve 10mg in 5ml of alcohol R, add 1g of Zn R and HCL R1, red color develops Assay: 95.0%~101.0% ( anhydrous substance, Titration with 0.1M N(C4H9)4OH ) Tests: Light-absorbing mpurities, at wave ength between 450nm and 800nm: 0.10max Substances insoluble in methanol: 3.0%max Related substances: Impurity A: Isoquercitroside : 2.0%max Impurity B: Kaempferol-3-rutinoside: 2.0%max Impurity C: Quercetin: 2.0%max Any other impurity: 0.1%max Total: 4.0%max Moisture: 7.5-9.5% Sulphated ash: 0.1%max Residual solvents: Methanol: 3000ppm Acetone: Absence Propyl alcohol: Absence Package: 25kg/ fibre drum