Calcein Blue

Storage: Ambient Temperature

Shipping Condition: Ambient Temperature

Chemical Name: 8-[N,N-Bis(carboxymethyl)aminomethyl]-4-methylumbelliferone

CAS Number: 54375-47-2

Appearance: white or pale yellow powder

Sensitivity: pass test

Sulfated ash: <0.5 %

Solubility: 1 g/25 ml, 1 M KOH100 ml water

Calcein Blue is a fluorescent metal indicator in EDTA titration. It is easily soluble in alkaline solutions, but barely soluble in water. Its proton dissociation constants are reported to be pKa1=2.45, pKa2=7.24 and pKa3=11.3. Calcein Blue solution emits bright blue

Appearance: white or pale yellow powder Sensitivity: pass test Sulfated ash: <0.5 % Solubility: 1 g/25 ml, 1 M KOHà100 ml water Calcein Blue is a fluorescent metal indicator in EDTA titration. It is easily soluble in alkaline solutions, but barely soluble in water. Its proton dissociation constants are reported to be pKa1=2.45, pKa2=7.24 and pKa3=11.3. Calcein Blue solution emits bright blue fluorescence (lem=445 nm) at pH 4-11, but no fluorescence at pH 12 or higher. The blue fluorescence of the Calcein Blue solution at pH 4-11 is quenched by metal ions such as Co, Cu, Mn, Ni and Pb. However, Calcein Blue forms a highly fluorescent complex with alkaline earth metals at pH 12 or higher. Since the endpoint of the chelate titration is clear by UV irradiation at 200-370 nm in a dark room, highly stained sample can be used. Calcein Blue is also suitable to determine Fe and Zr ions. Calcein Blue-Zn chelate can detect 10 ppb of F-. Calcein Blue aqueous solution emits laser at 449-490 nm.