LONZA Mycoplasma Detection Kit
Contact Info
- Add:北京市海淀区北太平庄路2号院2号楼1901室 --公司微信号：毕特博生物--业务负责人微信号：13366202681, Zip: 10008
- Contact: 张钰
- Tel:400-833-9299
- Email:1767180321@qq.com
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LONZAMycoplasma Detection Kit
(MycoAlert Mycoplasma Detection Kit)
Mycoplasma Detection Kit(MycoAlert Mycoplasma Detection Kit)Definition: Mycoplasma, also known as pleuropneumonia-like organisms (PPLO), are prokaryotes without cell walls and are currently recognized as the smallest and simplest prokaryotes. The only visible organelle in mycoplasma cells is the ribosome (mycoplasma are prokaryotic cells, and the only organelle in prokaryotic cells is the ribosome). They are resistant to many antibiotics, similar to pleuropneumonia-like organisms. I. Mycoplasma contamination has become a major issue in cell culture: 1. Studies show that over 15% of cultured cells worldwide are contaminated with mycoplasma. 2. Mycoplasma contamination can affect cells in multiple ways, including changes in metabolism, immune or biochemical characteristics, growth conditions, and cell survival. 3. Mycoplasma contamination can compromise the stability, reliability, and accuracy of experimental results. 4. Mycoplasma is difficult to detect and eliminate. Mycoplasma can easily pass through standard filters and resist most antibiotics, causing significant losses in cell culture. II. Routine testing is essential to effectively avoid mycoplasma contamination in cells: 1. Regular mycoplasma testing is the only way to ensure that cell culture remains unaffected by mycoplasma contamination. 2. Traditional mycoplasma detection methods require culturing samples on special selective media, often taking weeks, which is time-consuming, labor-intensive, and lacks sufficient sensitivity and specificity.
III. Detection Principle |
The Lonza MycoAlert Mycoplasma Detection Kit utilizes the specific enzymatic activity of mycoplasma for selective biochemical detection. The presence of these enzymes enables a rapid screening operation that sensitively detects mycoplasma contamination in samples. The viable mycoplasma are lysed, releasing enzymes that catalyze a reaction with the substrate provided in the kit, converting ADP to ATP. By measuring the ATP levels before and after adding the substrate, a ratio is obtained that indicates the presence of mycoplasma. If these enzymes are absent, there is no increase in the second reading compared to the first; if the enzymes react specifically with the substrate, it results in an increase in ATP levels. The rise in ATP is detected through a bioluminescent chemical reaction, as per the following formula: LuciferaseATP + Luciferin + O2 ——————> Oxyluciferin + AMPMg 2+ + PPi + CO2 + LIGHTThis reaction occurs at room temperature (18°C to 22°C), which is the optimal temperature for the luciferase reaction. In the detection principle of the mycoplasma detection kit, the final spectrophotometric reading shows that the intensity of light emitted is linearly related to the concentration of ATP, allowing for the detection of mycoplasma contamination. |
Product Features of the Lonza Mycoplasma Detection KitThe Lonza MycoAlert Mycoplasma Detection Kit is a classic kit specifically designed to detect mycoplasma contamination during cell culture. It includes all necessary reagents and an operational manual. This mycoplasma detection kit offers advantages such as simplicity of operation, speed, efficiency, stable and reliable results, and extremely high sensitivity, making mycoplasma detection easy and ensuring your cultures remain clean and uncontaminated. The main features of this kit are as follows: IV. Recommended: Lonza (formerly Cambrex) MycoAlert Mycoplasma Detection Kit: Utilizes luminescence detection to quickly reflect mycoplasma contamination in cell culture. Prevents damage caused by mycoplasma and saves effort. 1. Fast—Results can be obtained in just 20 minutes. 2. Sensitivity—Can detect very low levels of mycoplasma. 3. Simple—Just mix two reagents with your sample, and take two readings with a luminometer to get accurate results. 4. Complete—The kit includes all reagents needed for detection! V. Ordering InformationProduct CodeProduct NameSpecification |
----Mycoplasma contamination has become a major issue in cell culture!!
1. Studies show that over 15% of cultured cells worldwide are contaminated with mycoplasma.
2. Mycoplasma contamination can affect cells in multiple ways, including changes in metabolism, immune or biochemical characteristics, growth conditions, and cell survival.
3. Mycoplasma contamination can compromise the stability, reliability, and accuracy of experimental results.
4. Mycoplasma is difficult to detect and eliminate. Mycoplasma can easily pass through standard filters and resist most antibiotics, causing significant losses in cell culture.
-----Routine testing is essential to effectively avoid mycoplasma contamination in cells
1. Regular mycoplasma testing is the only way to ensure that cell culture remains unaffected by mycoplasma contamination.
2. Traditional mycoplasma detection methods require culturing samples on special selective media, often taking weeks, which is time-consuming, labor-intensive, and lacks sufficient sensitivity and specificity.
----Bitebo recommends the Lonza (formerly Cambrex) MycoAlert Mycoplasma Detection Kit!
Utilizes luminescence detection to quickly reflect mycoplasma contamination in cell culture. Prevents damage caused by mycoplasma and saves effort.
1. Fast—Results can be obtained in just 20 minutes.
2. Sensitivity—Can detect very low levels of mycoplasma.
3. Simple—Just mix two reagents with your sample, and take two readings with a luminometer to get accurate results.
4. Complete—The kit includes all reagents needed for detection!
Ordering Information:
LT07-118 | MYCOALERT DETECTION KIT- 10 TESTS | EA | ¥2,775 |
LT07-218 | MYCOALERT DETECTION KIT- 25 TESTS | EA | ¥5,410 |
LT07-318 | MYCOALERT DETECTION KIT- 100 TESTS | EA | ¥13,694 |
LT07-418 | MYCOALERT DETECTION KIT- 50 TESTS | EA | ¥9,122 |
LT07-518 | MYCOALERT ASSAY CONTROL SET-10 TESTS | EA | ¥2,675 |
Company: Beijing Bitebo Biotechnology Co., Ltd.------Authorized Distributor of LONZA in China
Contact: Zhang Yu
National Hotline: 400-833-9299
Phone: 13366202681
| Industry Category | Chemicals |
|---|---|
| Product Category | |
| Brand: | LONZA |
| Spec: | LT07-218 |
| Stock: | 100 |
| Manufacturer: | |
| Origin: | China / Beijing / Haidianqu |
