Silica gel powder chromatography packing analytical purification chromatography silica 300-400 mesh

Silica Gel Powder Chromatographic Filler Analytical Purification Chromatographic Silica 300-400 Mesh

Introduction to Column Chromatography Technique: Commonly referred to as "running a column," it should be called column chromatographic separation, also known as column chromatography. What we commonly use is an adsorption column with silica gel powder chromatographic filler or alumina as the stationary phase. In fact, silica gel column chromatography can also be referred to as silica gel adsorption column chromatography.

Chromatography, also known as chromatography, is a separation method based on the mechanism of distribution equilibrium. The chromatographic system consists of two phases: one is the stationary phase, and the other is the mobile phase. When the two phases move relative to each other, the differences in the distribution equilibrium properties of the components in the mixture are repeatedly utilized to achieve separation.

Related Applications of Chromatographic Silica Gel:

Extracting, separating, and characterizing small biomolecules from natural products is one of the primary pathways for obtaining lead compounds in new drug research and development. Chromatography is one of the commonly used methods for the separation of natural products. Initially, chromatography was used to separate plant pigments based on compound color. Today, it has evolved into a highly sensitive and effective method for the separation and identification of compounds. Column chromatography generally includes normal pressure column chromatography, medium pressure column chromatography, and high-pressure column chromatography. Based on column fillers, it can be further divided into column chromatographic silica gel, ion exchange chromatography, gel permeation chromatography, hydrophobic interaction chromatography, etc. Among these, column chromatographic silica gel (normal/reverse phase) is the most widely used. Compared to other chromatographic methods, column chromatography offers advantages such as simple operation, cost-effectiveness, and high sample loading capacity, making it one of the preferred methods for the separation and purification of natural products.