Introduction to Basic Information of Biological Buffer BES

BES is a white crystalline powder, also known as N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid. It is easily soluble in water and its aqueous solution has good pH buffering performance. Its molecular formula is C6H15NO5S, with a molecular weight of 213.25. The main uses of BES 1. Biological experiments: BES is one of the commonly used buffering agents in biological experiments, especially in environments where pH stability needs to be maintained. For example, when studying the structure and function of DNA, RNA, and proteins, BES can be used to stabilize pH values and ensure the accuracy of experimental results. 2. Cell culture: During the cell culture process, BES can provide a stable growth environment, which helps with cell growth and reproduction. It helps maintain the homeostasis of the intracellular environment and reduces the adverse effects of pH changes on cells. 3. Protein separation and purification: BES plays an important role in the process of protein separation and purification. It can effectively dissolve various biomolecules and salts, help stabilize the structure and properties of proteins, and improve protein recovery and purity. 4. Enzyme kinetics research: BES can be used as a buffer for enzyme kinetics research. Its stable pH environment helps to accurately measure enzyme activity and provides favorable conditions for studying enzymatic reactions. Advantages of BES 1. Stable pH range: BES has a wide pH range and can maintain stability between pH 6.2 and 8.0, providing reliable buffering for various biological experiments. 2. Low toxicity: Compared to other buffering agents, BES has lower toxicity to biomolecules, less impact on cells and enzymes, and reduces interference with experimental results. 3. High solubility: BES has high solubility and can effectively dissolve various biomolecules and salts, which is beneficial for experimental operations and solution stability. 4. Chemical stability: BES has stable chemical properties and is not easily reactive with other reagents, reducing errors caused by chemical reactions in experiments.