What is the function of the chromogenic substrate MADB 209518-16-1?

The Chinese name of MADB is N, N-di (4-sulfobutyl) -3,5-dimethylaniline sodium salt, with a CAS of 209518-16-1. It is a highly water-soluble and sensitive peroxidase chromogenic substrate commonly used in laboratories. It can react with peroxidase to form a complex, and this complex can achieve the purpose of detecting peroxidase activity through colorimetric reactions, thereby evaluating the immune function of cells, the proliferation ability of tumor cells, and the toxicity of environmental pollutants to organisms. Below, we will take you to analyze the role of MADB in detail. 1、 Substrate action The main function of MADB as a chromogenic substrate is to provide a reactive chemical structure to convert the reactive oxygen species generated by peroxidase enzymes into visible products. Because in this process, the structure of MADB has specific binding sites with the reaction products catalyzed by peroxides and enzymes, making the binding between substrates and enzymes tighter and more stable. 2、 Color change effect When MADB reacts with peroxidase, the substrate is oxidized into an unstable intermediate, which is then converted into a stable product through the reduction of MADB. During this process, the color of the substrate will change, which can be used to evaluate the activity and stability of peroxidase, as the more active the enzyme, the more intermediates and products it produces, resulting in a more pronounced change in substrate color. 3、 Signal amplification effect In addition to the role of the substrate itself, MADB can also enhance enzyme reaction efficiency through signal amplification. This is because the binding site between MADB and enzymes is highly specific, and only substrates that conform to a specific structure can react with it. Therefore, MADB has a high affinity and can be effectively catalyzed, precisely achieving this. In summary, as a peroxidase chromogenic substrate, MADB has multiple functions, which together constitute its unshakable position in color experiments. At the same time, it also provides researchers with a fast, sensitive and reliable method to help them complete complex experimental operations. As an advantageous supplier of color reagents, Desheng has developed a new type of Trinder's reagent that can be applied to various biochemical experimental projects. It not only has high purity, but also has sensitive and stable color development, and is cost-effective. If you have any relevant intentions, feel free to contact us for consultation and purchase!