Application of zwitterionic buffering agent CHAPSO

CHAPSO, as a zwitterionic buffering agent, is a dipole ion with both positive and negative charges in its molecular structure. It has a neutral charge, but the positively and negatively charged groups are different; In the gel electrophoresis experiment, it can completely destroy the cell structure, break the bonds between proteins, separate proteins, while maintaining the natural state and charge of a single protein. CHAPSO is commonly used in chromatography, 2D gel electrophoresis, mass spectrometry, and dissolution of organelles and proteins. CHAPSO, as a zwitterionic buffering agent, is a dipole ion with both positive and negative charges in its molecular structure. It has a neutral charge, but the positively and negatively charged groups are different; In the gel electrophoresis experiment, it can completely destroy the cell structure, break the bonds between proteins, separate proteins, while maintaining the natural state and charge of a single protein. CHAPSO is commonly used in chromatography, 2D gel electrophoresis, mass spectrometry, and dissolution of organelles and proteins. The CMC content of CHAPSO is very high, reaching 8mM, which is very suitable for use as a cleaning agent. It is also a neutral ion surfactant used as a biological buffer for dissolving biological macromolecules such as proteins in the laboratory. As CHAPSO is more suitable for dissolving endometrial proteins, it is used as a non denaturing detergent in protein purification to dissolve naturally occurring membrane binding proteins. A certain amount of CHAPSO can be mixed with succinylphosphatidylcholine to study membrane related proteins through solid-state nuclear magnetic resonance technology; Research has shown that in studying the dissolution and reconstruction process of transport protein liposomes, CHAPSO acts as a buffer to stabilize the pH value of the system within an appropriate range, ensuring the accuracy of the research results. The buffering ability of CHAPSO does not change significantly with temperature, so CHAPSO is a suitable buffering agent for studying new Bicell systems by directing large molecules over a wide temperature range.